Coding

Part:BBa_K3989010:Design

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)


plant immune elicitor flg22 fused with ClyA for OMV display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1105
    Illegal NgoMIV site found at 1099
    Illegal AgeI site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Flexible BsaI cutting site to make sure ClyA and flg22 are changable


Source

composite part

References